TABLE 1.

Physical characteristics of PLGA nanoparticles

Nontargeted HA-ts-HT- and targeted HA-ts-HT-CPE-loaded nanoparticles were prepared with or without plx. The diameter of the particles in solution and P.D.I. were measured by Zetasizer Nano (ZS90) by a dynamic light scattering method. The total % protein loading (w/w) and % surface loading from total loading was measured using BCA protein assay. Control experiments revealed that there is negligible interference by blank PLGA particles on BCA assay. Percent protein (% w/w) loading for blank particles was ∼0.031%. The zeta potential in PBS at pH 7.4 was measured by Zetasizer Nano (ZS90). The particle size, % protein loading, % surface loading, and zeta potential are expressed as mean ± S.D. for three different preparations of nanoparticles.

Sample	Particle diameter	P.D.I.	% protein loading	% surface loading from total loading	Zeta potential in PBS, pH 7.4
	nm		w/w		mV
HA-ts-HT	464 ± 12	0.11	2.02 ± 0.39	15.25 ± 1.40	−2.46 ± 0.67
HA-ts-HT-CPE	449 ± 17	0.08	2.16 ± 0.38	16.80 ± 1.08	−2.34 ± 0.19
HA-ts-HT + plx	512 ± 12	0.11	1.62 ± 0.33	17.77 ± 4.24	−2.08 ± 0.60
HA-ts-HT-CPE + plx	449 ± 21	0.10	1.76 ± 0.28	15.45 ± 1.04	−1.66 ± 0.34