FIGURE 3.

The PAK4-binding integrin β5 tail SERS-motif regulates cell attachment and migration. A and B, the expression levels of CS-1 cells transiently transfected with integrin β5-WT or co-transfected with integrin β5-WT and FLAG-PAK4-WT analyzed by immunoblotting (A) or flow cytometry (B) with untransfected CS-1 cells as negative control and actin as a loading control. Percentages and M1 values are as shown as in Fig. 2. C, cell attachment and (D) cell motility on VN (10 μg/ml) of CS-1 cells transiently transfected with β5-WT or co-transfected with integrin β5-WT and PAK4-WT. E, cell attachment and (F) cell motility on VN of stable clones of CS-1-β5-WT, CS-1-β5-ER760,761RE, and CS-1-β5-ΔSERS transiently co-expressing EGFP (open bars) or EGFP-PAK4 (solid bars). Parental CS-1 cells transfected with EGFP or EGFP-PAK4 served as background control (not shown). Values obtained with CS-1-β5-WT co-transfected with EGFP empty vector were defined as control. Bars represent mean values ± S.E. (n = 3). Statistically discernable differences as determined by t test are indicated (*, p ≤ 0.05; **, p ≤ 0.01). Displayed results are representative of three or more experiments.