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Addition of serine residues to the cytosolic tail of TCRα enhances its ubiquitination and degradation. A and B, ubiquitination of WT TCRα and serine-addition mutants of TCRα (Fig. 1A) was determined as described in the legend to Fig. 1, B and C. *, p < 0.05 versus WT. C and D, CHX chase experiments and quantification were performed as described in the legend to Fig. 1, D and E.
