FIGURE 7.

Dose dependence of resveratrol remodeling of Aβ amyloid fibrils. Aβ fibrils (25 μm) were incubated with resveratrol (Res.) or control solutions for 4 h and monitored using SDS-PAGE (A), cell toxicity analysis (2.5 μm Aβ; n = 3) (B), dot blot analysis using both conformation-specific (OC) and sequence-specific (6E10) antibodies (C), and ThT (n = 5) (D). In A, Aβ fibril samples (both before and after incubation with resveratrol) were also heated (100 °C, 10 min) as loading controls. Van., vanillin; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; RFU, relative fluorescence units.