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. 2010 Jul 28;5(7):e11821. doi: 10.1371/journal.pone.0011821

Figure 6. Repression of AES mediated TCF/LEF-1 transcription by nuclear LRP6-ICD.

Figure 6

HEK 293T cells were transfected with luciferase assay reporter constructs (TOP reporter) along with (A) control LacZ, control LacZ and AES-HA (AES) or AES-HA and GFP-LRP6-ICD (ICD) for 24 hrs (B) control LacZ and control pcDNA-NES, control pcDNA-NES and AES-HA or AES-HA and GFP-LRP6-ICD-NES (ICD-NES) for 48 hrs (C) control LacZ and control EV-GFP-NLS, control EV-GFP-NLS and AES-HA or AES-HA and GFP-LRP6-ICD-NLS (ICD-NLS) for 24 hrs and TCF/LEF-1 activity was measured. TCF/LEF-1 activity is expressed as a percentage of the relevant control(s) used in each condition: (**P<0.001).