Table 2.
Changes in protein expression between wild-type and SP-A-/- mice for control, 4 hr post infection and 24 hr post infection: percent changes with significance for all identified proteins corresponding to reference gels in Fig. 2
| Gel No. | Protein | Functional Group | % Δ WT to SP-A-/- Baseline |
% Δ WT to SP-A-/- 4 hr |
% Δ WT to SP-A-/- 24 hr |
|---|---|---|---|---|---|
| 1 | 14-3-3-Zeta | DEF, RED, PMM | -34.97 | 16.24 | -10.69 |
| 2 | Adipsin (Complement factor D) | DEF, PMM | -63.51 | 5.95 | 4.76 |
| 3 | Albumin | RED | 4.82 | 1.56 | 12.73 |
| 4 | Aldehyde dehydrogenase AHD-M1 | RED | -82.62 | 37.46 | 29.21 |
| 5 | Aldehyde dehydrogenase II | RED | 135.85 | 14.63 | 31.75 |
| 6 | Aldehyde dehydrogenase, Dimeric NADP-preferring (EC 1.2.1.5) (ALDH class 3) | RED | 58.04 | 40.10 | 38.77 |
| 7 | Alpha-1-antitrypsin 1-1 precursor (Serine protease inhibitor 1-1) | DEF, PMM | -30.98 | 15.12 | 16.63 |
| 8 | Alpha-1-antitrypsin 1-6 precursor (Serine protease inhibitor 1-6) (Alpha-1 protease inhibitor) | DEF, PMM | -12.72 | -9.38 | -12.84 |
| 9 | Alpha-fetoprotein | -16.1 | 24.43 | -11.61 | |
| 10 | Annexin A1 (Annexin I) (Lipocortin I) (Calpactin II) (Chromobindin-9) (P35) | DEF | -201.08 | 72.45 | 4.89 |
| 11 | Annexin A3 | 21.76 | -7.73 | 1.22 | |
| 12 | Annexin A4 | 13.78 | 14.08 | 3.38 | |
| 13 | Annexin A5 | DEF | -381.16 | -66.73 | -130.01 |
| 14 | Apolipoprotein A-1 | DEF, RED | -23.3 | -89.53 | 93.80 |
| 15 | Beta-2-microglobulin | DEF | 68.18 | -59.79 | -55.69 |
| 16 | Beta-actin | -14.27 | 32.95 | 13.93 | |
| 17 | Beta-actin (putative, AA 27-375) (alpha-actin) | 4.84 | 52.39 | 9.19 | |
| 18 | Carbonyl reductase 2 | RED | -184.18 | 29.93 | 0.81 |
| 19 | Cell specific 10K protein (uteroglobin - mouse) (Clara cell 10K protein) (CC10) (CC16) | DEF, RED | -73.76 | 12.90 | 64.31 |
| 20 | Ceruloplasmin isoforms | RED | 22.02 | -9.13 | -19.71 |
| 21 | Chain A, Crystal structure of novel mammalian lectin Ym1-suggests a saccharide binding site | DEF | -2.98 | 28.89 | 13.35 |
| 22 | Chain B, Chimeric human mouse carbonmonoxyhemoglobin (Human zeta, Mouse beta 2) | -5.61 | 8.67 | 33.51 | |
| 23 | Chia protein | DEF | 5.35 | 31.40 | 17.25 |
| 24 | Coiled-coil domain containing 122 | -183.85 | 21.31 | -6.43 | |
| 25 | Complement component 3 | DEF | -12.5 | 40.66 | 8.39 |
| 26 | Complement component C5 | DEF | -22.76 | 21.75 | 5.19 |
| 27 | Contrapsin (Serine protease inhibitor A3K) | PMM | 7.77 | -8.52 | -8.74 |
| 28 | Creatine kinase M-type (EC.2.7.3.2) (Creatine kinase M chain) (M-CK) | -57.22 | 28.34 | -22.83 | |
| 29 | Cytosolic malate dehydrogenase | RED | -3.05 | 7.03 | 1.31 |
| 30 | (Similar to) Ferritin light chain 1 (Ferritin L, subunit 1) | DEF, RED | -87.39 | -14.84 | -3.35 |
| 31 | Gamma-actin | -20.73 | 31.85 | 20.75 | |
| 32 | Gelsolin precursor (Actin-depolymerizing factor) (ADF) (Brevin) | DEF, RED, PMM | 30.02 | -3.09 | -2.90 |
| 33 | Glutathione S-transferase, alpha 3 | DEF, RED | -193.11 | 21.05 | 19.07 |
| 34 | Glutathione S-transferase, alpha 4 | DEF, RED | -339.42 | 64.33 | 38.47 |
| 35 | Glutathione S-transferase, mu 1 | DEF, RED | -101.12 | 45.92 | 35.09 |
| 36 | Glutathione S-transferase, omega 1 | DEF, RED | -132.89 | 48.91 | 15.84 |
| 37 | (Similar to) Glutathione S-transferase, Ya chain (GST class-alpha) (Ya1) | DEF, RED | -318.76 | 56.63 | 44.05 |
| 38 | Haptoglobin | DEF, RED | -14.69 | 16.92 | 2.41 |
| 39 | Hemoglobin subunit alpha (Hemoglobin alpha chain)(Alpha-globin) | -6.6 | -32.60 | 132.59 | |
| 40 | Isocitrate dehydrogenase [NADP] cytoplasmic (EC 1.1.1.42) (Cytosolic NADP-Isocitr) | RED | -21.59 | 17.31 | 1.14 |
| 41 | Keratin complex 1, acidic, gene 10 | 16.11 | 20.63 | -30.55 | |
| 42 | (Similar to) Keratin, type I cytoskeletal 10 (Cytokeratin-10) (CK-10) (Keratin-10) | -43.31 | 26.68 | -5.45 | |
| 43 | Kpnb1 protein b | -55.63 | 53.13 | 34.52 | |
| 44 | Lactate dehydrogenase 2, B chain | DEF, RED | 28.46 | -4.34 | -3.26 |
| 45 | Lysozyme 2 | DEF, RED | 129.29 | -63.56 | -133.43 |
| 46 | Murinoglobulin-1 precursor (MuG1) | DEF, PMM | -14.21 | -16.51 | -6.93 |
| 47 | Myosin heavy chain IIB | -21.97 | 6.81 | -15.67 | |
| 48 | Peroxiredoxin 1 | RED | -83.18 | 24.93 | 11.30 |
| 49 | Peroxiredoxin 6 | DEF, RED | -54.85 | 56.63 | 42.36 |
| 50 | Pregnancy zone protein | DEF, PMM | -1.56 | 2.46 | -7.38 |
| 51 | Prothrombin precursor (Ec 3.4.21.5) (Coagulation factor II) | DEF, PMM | -59.43 | 6.39 | 5.13 |
| 52 | Pulmonary surfactant associated protein A precursor (SP-A) (PSP-A) (PSAP) | DEF, RED | ND | ND | ND |
| 53 | Rho GDP dissociation inhibitor (GDI) alpha | -115.4 | -22.62 | -42.11 | |
| 54 | SEC14-like 3 | PMM | -33.59 | 30.46 | 9.92 |
| 55 | Selenium binding protein 1 | DEF, RED | 9.26 | -4.10 | -3.32 |
| 56 | Selenium binding protein 2 | DEF, RED | 27.17 | -2.40 | -3.86 |
| 57 | Serine (or cysteine) proteinase inhibitor, clade A, member 1e | PMM | 7.7 | -18.15 | -11.72 |
| 58 | Toll-like receptor 13 precursor | DEF | 18.41 | -17.04 | -4.57 |
| 59 | Transferrin | DEF, RED, PMM | 8.65 | -17.12 | -0.37 |
| 60 | Tyrosine-3-monooxygenase/tryptophan 5-monooxynase activation protein, Epsilon polypeptide | PMM | -183.11 | 18.41 | -7.70 |
Changes (% Δ) in BAL protein expression from wild-type (WT) to SP-A-/- mice are shown for untreated (baseline) animals, 4 hours post-infection and 24 hours post-infection. Gel numbers (see Fig. 2), protein names, functional groups and percent change (% Δ) are listed. Abbreviations for functional groups: DEF, host defense; PMM, protein modification and metabolism; RED, redox regulation. Bolded numbers indicate changes that were significant (p < 0.05 by t-test). Values for SP-A, which is absent in SP-A-/- mice, are listed as not determined (ND).