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Met is not amplified, but is overexpressed at the protein level. (A) Lysates from the indicated breast cancer cell lines was prepared and 100 ug of protein was separated by SDS-PAGE, transferred, and immunoblotted with anti-EGFR, anti-Met, and anti-β-actin. (B) Lysates from the Met expressing breast cancer cell lines were immunoprecipitated with anti-Met antibodies. Immunoprecipitates were separated by SDS-PAGE, transferred, and immunoblotted with anti-pMet antibodies.
