Figure 4.

Decreased proliferation but normal survival of rictor-deficient T cells. (A) Splenocytes of WT and rictor cKO mice assayed for ³H-thymidine incorporation 48 h after activation with αCD3 + αCD28 (representing three replicate experiments). (B) As in (A), except that cells were analyzed by flow cytometry for BrdU incorporation (left panel) or division history using CFSE partitioning (right panel). Shown are histograms of events in the CD4⁺ and viable lymphoid gates from one experiment representative of three replicates. (C) Mean (±SEM) from one of two replicate experiments measuring viable CD4⁺ T cell numbers after triplicate cultures (1–3 d) with or without IL-4, expressed as a percentage of the input viable cell counts. (D) Spleen cells were cultured (20 h) in the presence or absence of IL-4 and assayed by TUNEL. Shown are flow cytometry profiles for CD4⁺ cells; numbers denote % TUNEL positive cells in one experiment representative of 4 replicates. (E) Cells were irradiated, cultured in the presence or absence of IL-4, and assayed by TUNEL. Additional information is in supplemental Fig. S3.