Figure 3. Lack of InsP3R Ca2+ Release Activity Actives Pro-Survival Mechanisms.
(A and B) Glucose (A) and glutamine (B) consumption measured daily in DT40 cells. (C) Cytochalasin B (Cyt B)-sensitive deoxyglucose uptake by WT and KO cells (n = 3). (D) Effects of autophagy inhibition by 3MA in nutrient-rich media on viability (PI-negative cells) of WT and KO cells after 24 hr (n = 3). (E and F) Survival of WT and KO cells in starvation conditions (Hank’s) in absence (E) and presence (F) of 3MA (n = 3). (G) Death (PI-positive cells) of Atg5-deficient primary hepatocytes induced by XeB (n = 3). (H) Recovery of WT and KO cells after 24 hr starvation. Same number of cells seeded in complete medium (n = 3). Error bars shown when bigger than points. *** p < 0.001, ** p < 0.01.