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. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: J Mol Biol. 2009 Mar 12;388(2):299–309. doi: 10.1016/j.jmb.2009.03.012

Figure 2.

Figure 2

The interaction FcsSBP with carbohydrates. A) The UV difference spectrum induced by the binding of the A-trisaccharide to FcsSBP. Peaks and troughs in the spectrum are labelled. B) An isotherm of FcsSBP binding to the A-trisaccharide produced by a UV difference titration. Each curve corresponds to a different peak-to-trough wavelength pair used to measure the change in UV absorbance upon ligand binding: triangles, 287.0-282.6 nm pair; circles, 293.7-290.0 nm pair; and squares, 287.0–290.0 nm pair. Error bars show the standard deviation of triplicate measurements. Solid lines show the fits to a one-site binding module accounting for ligand depletion. C) Representative isothermal titration calorimetry titration for the A-trisaccharide. The upper panel shows the unintegrated data corrected for baseline. The lower panel shows the integrated heats fit to a one-site binding model.