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. Author manuscript; available in PMC: 2010 Jul 28.
Published in final edited form as: Mol Microbiol. 2010 May 19;77(1):183–199. doi: 10.1111/j.1365-2958.2010.07199.x

Figure 5.

Figure 5

Analysis of maltooligosaccharides in the culture supernatants of S. pneumoniae grown in the presence of glycogen. A) Upper trace, oligosaccharides present in the culture supernatant of wild-type S. pneumoniae TIGR4 grown in ACGHY media with glycogen; middle trace, oligosaccharides present in the culture supernatant of the Δspua strain grown in the same medium; lower trace, oligosaccharides present in the culture supernatant of the Δmalx strain. B) Upper trace, ACGHY media containing glycogen, supplemented with recombinant SpuA (indicated as rSpuA) and incubated as for other cultures but not inoculated with bacteria. Second trace, oligosaccharides present in the culture supernatant of wild-type S. pneumoniae TIGR4 grown in ACGHY media with glycogen and supplemented with recombinant SpuA. Third trace, oligosaccharides present in the culture supernatant of Δspua grown in ACGHY media with glycogen and supplemented with recombinant SpuA. Lower trace, oligosaccharides present in the culture supernatant of the Δmalx strain grown in ACGHY media with glycogen and supplemented with recombinant SpuA. In all panels, oligosaccharide identities for maltooligosaccharides with degrees of polymerization from 2 to 8 were determined from comparison to known standards; these are labelled M2-M8. Oligosaccharides M9-M28 are inferred to be α-1,4-glucooligosaccharides with degrees of polymerization from 9 to 28.