Table 1.

Proteins encoded by the Streptococcus pneumoniae TIGR4 genome that are putatively involved in α-glucan metabolism.

Protein Name	Locus Tag	CAZy Familya	Cellular location (PSORTf)	Putative virulence factord	Putative activity
MalA	SP2111	N/A	Cytoplasm		Unknown
MalR	SP2112	N/A	Cytoplasm		Negative regulator
MalX	SP2108	N/A	Membrane/cell wall	✓	Solute binding
MalC	SP2109	N/A	Membrane		ABC transporter - permease
MalD	SP2110	N/A	Membrane		ABC transporter - permease
MalP	SP2106	GT35b	Cytoplasm		Glycogen phophorylase
MalM	SP2107	GH77c	Cytoplasm		4-α-glucanotransferase
GlgA	SP1124	GT5	Cytoplasm		Glycogen synthase
SpuA	SP0268	GH13(12)e	Membrane/cell wall	✓	pullulanase
SpAMY	SP1382	GH13(5)	Cytoplasm	✓	α-amylase
AG13	SP1883	GH13(29)	Cytoplasm	✓	α-phosphotrehalase
Dex	SP0342	GH13(31)	Cytoplasm		α-1,6-glucosidase
Pul	SP1118	GH13(14)	Cytoplasm	✓	pullulanase
SP	SP1894	GH13(18)	Cytoplasm		Sucrose phosphorylase
GT	SP1121	GH13(9)	Cytoplasm		1,4-α-glucan branching enzyme
AG31	SP0312	GH31	Cytoplasm	✓	α-glucosidase

^asee URL: http://www.CAZY.org (Cantarel et al., 2008).

^bthe prefix GT refers to the glycosyltransferase families

^cthe prefix GH to the glycoside hydrolase families.

^das identified by signature tagged mutagenesis (Hava & Camilli, 2002) and/or microarray analysis (Orihuela et al., 2004).

^enumbers in brackets refers to the subfamily of GH13, which was used to aid in the functional prediction (Stam et al., 2006).

^f(Gardy et al., 2005)