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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Neurogastroenterol Motil. 2010 May 13;22(8):909–e237. doi: 10.1111/j.1365-2982.2010.01508.x

Figure 4.

Figure 4

Photomicrographs of immunoreactivity in the myenteric plexus of guinea pig ileum. [A]: Example images of calbindin immunoreactivity in the myenteric plexus of neonatal ileum. [B]: Example images of Chat immunoreactivity in the myenteric plexus of neonatal ileum. [C] and [D]: VAChT-ir in ileal LMMP made from neonatal and adult guinea pig. Based on descriptive comparison by blinded observers, density of VAChT-ir in myenteric neurons in neonatal tissue [C] was higher than it was in adult tissue [D]. [E] and [F]: nAChRs-ir in ileal LMMP made from neonatal and adult guinea pig. Based on descriptive comparison by blinded observers, density of nAChRs -ir in myenteric neurons in neonatal tissue [E] was not different from it was in adult tissue [F]. In [C], [D], [E] and [F], tissues were fixed, processed, stained and camera-captured under the same conditions. Scale bars: 15 µm.