Table 1.
Half maximal effective concentrations (EC50) and Hill slope values for ACh and ATP concentration response curves in HEK-293 cells expressing nAChRs and P2X2 receptors individually and together.
| EC50 (μM) | Hill slope | |
|---|---|---|
| nAChR stable (n = 9) | 172 ± 31 | 1.6 ± 0.2 |
| nAChR stable with transient P2X2 (n = 6) | 471 ± 176* | 1.4 ± 0.3 |
| P2X2 stable (n = 4) | 34 ± 7 | 0.8 ± 0.2 |
| P2X2 stable with transient nAChRs (n = 4) | 72 ± 7* | 1.9 ± 0.3* |
Data are mean ± s.e.m. “n” values are the number of cells.
*
indicates significantly different from value obtained in cells expressing on one receptor.