Fig. 4.

Nanotraps for biochemical applications. a V_HH-based nanotraps. b Example of the use of a GFP-specific nanotrap immunoprecipitation of GFP–profilerating cell nuclear antigen (PCNA) (approximately 60 kDa) from a crude cell lysate of GFP-PCNA expressing HEK 293T cells. Input (I) and bound (B) fractions were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by Coomassie staining. The GFP-Trap facilitates specific purification of GFP fusion proteins and their interaction partners