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. 2009 Jul 31;43(1):26–34. doi: 10.1165/rcmb.2009-0073OC

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Figure 8. — Effects of actin disruption with 0.1 μM cytochalasin D (Cyt D) on F₃₄₀/F₃₈₀ ratio induced by 1 μM thapsigargin or 10 U/ml thrombin. (A) Representative trace of F₃₄₀/F₃₈₀ ratio by stretching to 30% strain, and 1 μM thapsigargin after treatment with 0.1 μM cytochalasin D for 15 min After extracellular solution was changed to Ca²⁺-free solution for 40 s, 2 mM Ca²⁺ was re-added to the solution. (B) Levels of F₃₄₀/F₃₈₀ ratio in response to thapsigargin before (1st) and after (2nd) exposing Ca²⁺-free solution, with (Control) or without 0.1 μM cytochalasin D treatment. Bars represent means ± SD (n = 5). (C) Representative trace of F₃₄₀/F₃₈₀ ratio induced by 30% strain and 10 U/ml thrombin after treatment with 0.1 μM cytochalasin D for 15 min. (D) Levels of F₃₄₀/F₃₈₀ ratio in response to 10 U/ml thrombin with (Control) or without 0.1 μM cytochalasin D treatment. Bars represent means ± SD (n = 5).