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Growth factors activate MAPKs in CCL-151. At time zero, a volume equal to that already bathing the cells was added with EGF, FGF2 (basic FGF), or PDGF-BB at twice the final concentration of 100 ng/mL (three wells per plate). Untreated wells (three wells per plate) received an equal amount of fluid. Cell lysates were collected for analysis of MAPK phosphorylation.
