Figure 1.

The effect of tilapia ghrelin-C8 and ghrelin-C10 on brain NPY mRNA levels. Two different doses of both ghrelins, low (1 ng/gm) and high (10 ng/gm), were administered by a single i.p. injection and samples were collected a 2, 4, and 8 h postinjection. mRNA levels were normalized to the house keeping gene, acidic ribosomal phosphoprotein P0 (ARP). Vertical bars represent mean ± SEM (n = 8–10). ∗, ∗∗ are significantly different from time-matched control at P < .05 and <.01, respectively (2-way ANOVA).