Figure 2.

Negative ion mode electrospray mass spectra of mixtures of 10 μM telomestatin and 5 μM of G-quadruplexes. Zooms on the peaks of free G-quadruplex (a) [TG₄T]₄, (b) telomeric sequence (T₂AG₃)₄, and (c) Pu22myc promoter sequence GAG₃TG₄AG₃TG₄A₂G, and on the complexes between (d) one telomestatin and [TG₄T]₄, (e) one telomestatin and (T₂AG₃)₄, and (f) one telomestatin and GAG₃TG₄AG₃TG₄A₂G. The spectra were recorded from and 80/20 aqueous ammonium acetate (150 mM)/methanol solution, using soft source conditions to preserve the specifically bound ammonium ions.