S. No. | Ionic strength of KBPES buffer (pH7.0) | Stoichiometry DNA : drug | Percentage of fluorescence hypochromicity |
---|---|---|---|
1. | TMPyP4 with 27BCL2 | ||
1.a | 30 mM | 1 : 4 | 34.2% |
1.b | 100 mM | 1 : 4 | 28.5% |
1.c | 200 mM | 1 : 4 | 20.3% |
1.d | 600 mM | 1 : 4 | 6.5% |
| |||
2. | TMPyP3 with 27BCL2 | ||
2.a | 30 mM | 1 : 2 | 59.8% |
1 : 3 | 51.5% | ||
2.b | 100 mM | 1 : 2 | 52.5% |
1 : 3 | 49.5% | ||
2.c | 200 mM | 1 : 2 | 33.6% |
1 : 3 | 16.6% | ||
2.d | 600 mM | 1 : 2 | 8.6% |
1 : 3 | 4.9% | ||
| |||
3. | TMPyP2 with 27BCL2 | ||
3.a | 30 mM | 1 : 2 | 37.4% |
1 : 3 | 40.2% | ||
3.b | 100 mM | 1 : 2 | 11.9% |
1 : 3 | 12.3% | ||
3.c | 200 mM | 1 : 2 | 6.5% |
1 : 3 | 4.4% | ||
3.d | 600 mM | 1 : 2 | 0.7% |
1 : 3 | 0.3% |