Effect of Ionic Strength on Porphyrin Drugs Interaction with Quadruplex DNA Formed by the Promoter Region of C-myc and Bcl2 Oncogenes

. 2009 Oct 18;2010:146418. doi: 10.4061/2010/146418

(b) Effect of ionic strength on the percentage of fluorescence hypochromicity while C-myc interacts with porphyrin drugs.

S. No.	Ionic strength of KBPES buffer (pH7.0)	Stoichiometry DNA : drug	Percentage of fluorescence hypochromicity
1.	TMPyP4 with C-myc
1.a	30 mM	1 : 4	39.2%
1.b	100 mM	1 : 4	32.6%
1.c	200 mM	1 : 4	29.3%
1.d	600 mM	1 : 4	5.5%

2.	TMPyP3 with C-myc
2.a	30 mM	1 : 2	52.8%
2.a	30 mM	1 : 3	49.5%
2.b	100 mM	1 : 2	47.5%
2.b	100 mM	1 : 3	42.2%
2.c	200 mM	1 : 2	29.6%
2.c	200 mM	1 : 3	12.6%
2.d	600 mM	1 : 2	6.6%
2.d	600 mM	1 : 3	3.9%

3.	TMPyP2 with C-myc
3.a	30 mM	1 : 2	42.4%
3.a	30 mM	1 : 3	37.2%
3.b	100 mM	1 : 2	12.6%
3.b	100 mM	1 : 3	7.2%
3.c	200 mM	1 : 2	7.8%
3.c	200 mM	1 : 3	5.4%
3.d	600 mM	1 : 2	0.6%
3.d	600 mM	1 : 3	0.2%