Figure 1. Naive and memory phenotype alloreactive CD8 T cells are detectable in LCMV-immune mice.

Splenocytes from LCMV-immune B6 mice (A, B and D) or naïve B6 mice (C) were stimulated with peptides or with splenocytes (allogeneic, irradiated LPS-treated cells) that were either syngeneic (H2^b) or allogeneic (BALB/c, H2^d or CBA, H2^k), as described in Materials and Methods. After culture, splenocytes were stained for cell surface markers and for intracellular IFN–γ and TNF. For analysis, samples were gated on CD8⁺ cells, and the values shown represent the percentage of CD8 T cells staining positive for either cytokine. The data are representative of 4 experiments.