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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Am J Transplant. 2010 Aug;10(8):1870–1880. doi: 10.1111/j.1600-6143.2010.03073.x

Figure 6.

Figure 6

Kinetics of alloreactive CD4+ T cells in islet recipients before (day 0) and at various times after transplant. Cryopreserved, patient-derived PBMC from the specified timepoints were batch analyzed for alloreactivity by intracellular IFNγ staining and flow cytometry. Patient PBMC were either incubated with irradiated patient-specific donor splenocytes or an irradiated pool of 20 random blood donors (3rd party allostimmulator). The % of CD4+ T cells producing IFNγ following 5 days of culture was measured. The IFNγ index represents the % of CD4+ T cells producing IFNγ in response to donor splenocytes) divided by the % of CD4+ T cells producing IFNγ in response to the 3rd party allostimmulator.