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. 2010 Jul 12;190(1):25–34. doi: 10.1083/jcb.201002133

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© 2010 Hewitt et al.

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Figure 1. — AZ3146, a novel Mps1 inhibitor. (A) AZ3146 (AZ) is shown. (B) In vitro kinase assay measuring the ability of Mps1^Cat to phosphorylate myelin basic protein (MBP). (C) In vitro kinase assays measuring the activity of Mps1 immunoprecipitated from nocodazole-arrested HeLa cells. (D) Dose–response curve showing inhibition of Mps1^Cat by AZ3146. (E) Activity of AZ3146 against a kinase panel. (F) Phos tag immunoblot of Mps1 isolated from interphase cells or nocodazole-arrested cells treated with MG132 ± 2 µM AZ3146 for 2 h. Arrowhead, major band; asterisk, minor band. Error bars indicate SEM derived from three experiments.