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. 2010 Jul 12;190(1):35–43. doi: 10.1083/jcb.201004017

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© 2010 Rankin and Wordeman

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Figure 3. — Taxol treatment causes severe spindle rocking and long astral MTs. (A) Representative HeLa cell coexpressing EGFP–myosin IIA and mCherry–α-tubulin with severe spindle rocking treated with 10 µM taxol added 80 s after anaphase onset. (B) 45 ± 2% of cells treated with 10 µM taxol exhibited spindle rocking compared with no DMSO-treated cells. (C) HeLa cells treated with 10 µM taxol for 1 min have longer astral MTs than cells treated with DMSO. Astral MTs in DMSO-treated cells do not contact the cortex (top inset), whereas MTs in taxol-treated cells come in close contact with the cortex (bottom inset). Insets show higher magnification views of boxed areas. Error bars indicate SEM.