Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jul 12;190(1):35–43. doi: 10.1083/jcb.201004017

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Rankin and Wordeman

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — The polar actin cortex is comparatively diminished during late anaphase. (A) Live cell expressing GFP-Utr (green) and mRFP-EB3 (red) in metaphase (left) versus anaphase (right). The polar actin cortex (arrow), equatorial actin cortex (closed arrowhead), and deep actin filaments (open arrowhead) are indicated. (B) Quantification of polar versus equatorial cortical actin fluorescence (arbitrary units) in metaphase versus anaphase. Polar actin fluorescence decreases in parallel with an increase in equatorial actin before cytokinesis (P < 0.0001). (C) Successive frames of a live, MCAK-depleted cell expressing GFP-Utr and RFP-EB3. A bleb forms (asterisk), is invaded by MT ends, and widens. (D) The same cell at the onset of severe spindle rocking (arrows) is shown. (E) Kymograph of the same MCAK-depleted cell showing the temporal position of the first large bleb (asterisk). t, time; d, distance. (F) Diagram illustrating how blebs lead to rocking in MCAK-depleted cells. A small bleb forms (left) and is rapidly invaded with MT polymer, leading to a shift in the spindle position and an increase in the size of the bleb (middle). Resolution of the large bleb leads to a large reversal of the spindle position in the opposite direction (right). Error bars indicate SEM.