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. 2010 Jul 12;190(1):89–100. doi: 10.1083/jcb.201001050

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© 2010 Maciejowski et al.

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Figure 4. — Mps1 protects cyclin B and securin from premature destruction. (A) Cells of the indicated genotypes were treated with or without 3MB-PP1, fixed, and stained with antibodies to serine 10–phosphorylated histone H3 (pH3) and securin (left) or cyclin B (right). Nuclei were counterstained with DAPI. Prophase cells were identified by their uniform pH3 staining (Hendzel et al., 1997), partially condensed chromatin (left), and nuclear import of cyclin B (right; Hagting et al., 1999). Note that cyclin B staining used methanol fixation, which preserves chromatin condensation less well than aldehyde fixation. (B) Cells were treated with both 3MB-PP1 and MG132 for 2 h and analyzed as in A. Asterisks indicate statistically significant deviation (P < 0.01 by one-way analysis of variance) from Mps1^wt cells treated with 3MB-PP1. Error bars indicate SEM. Bars, 10 µm.