Figure 6. Specific inhibition of nuclear accumulation by the specific CaM antagonist CDZ of HMG-containing proteins.

(A) Alignment of SRY, SOX2, SOX9, SOX10 and Nhp6Ap (NHP6A) HMG-box domains, along with the HMGN1 nucleosomal binding protein. The text in red represents conserved residues in the HMG domains of the SOX proteins and the residues in the grey box are the potential CaM-binding domains. (B) HeLa cells were transiently transfected to express the indicated GFP-fusion proteins, treated with or without 5 μM CDZ 16 h p.t. and imaged 8 h later by CLSM using a 40× water-immersion objective. (C) Results of quantitative analysis, whereby images such as those in (B) were analysed as described in Figure 1(C). Results are mean±S.E.M. (n>30) from a single typical experiment, from a series of three similar experiments; P values are indicated for Fn/c values that were significantly different in the presence of CDZ.