Table 1. Summary of properties of the sex-reversing NLS-mutation-containing SRY-HMG proteins used in the present study.

ND, not determined.

	Effect on DNA*				Ability to bind‡
SRY mutant	Binding	Bending	Effect on nuclear accumulation†	CDZ sensitivity†	CaM	Impβ1
WT	High affinity	Bends DNA 66 °	Accumulates strongly in the nucleus	Reduced 2-fold	High affinity	High affinity
M64T	WT activity	WT activity	Reduced 2–3-fold	None	Reduced ~5-fold	High affinity
F67V	Reduced >99.9%	ND	Reduced ~3-fold	None	Reduced 3-fold	High affinity
R76P	WT activity	WT activity	Reduced ~1.5-fold	None	Reduced ~3-fold	High affinity
Y127C	No activity	ND	Reduced ~2-fold	Reduced 2-fold	High affinity	Reduced 4-fold
R133W	WT activity	WT activity	Reduced ~2-fold	Reduced ~2-fold	ND	Reduced 2-fold

*From [9,10]; the R76P mutant has been reported to have very slightly reduced DNA-binding/bending abilities [10].

†Results from transfection studies using GC-2 cells (Figures 2B and 4D).

‡Results from AlphaScreen®/native PAGE assays (see Table 3); see also [10].