Figure 8. Effect of HOSCN exposure on gene expression in J774A.1 cells.

MAPK gene array following treatment of J774A.1 cells with HOSCN. Cells (plated at 0.5×10⁶ ml⁻¹) were exposed to 50 μM HOSCN (open bars) or 100 μM HOSCN (solid bars) in PBS at 37 °C for 15 min. Total RNA was subsequently extracted, used to synthesize cDNA and used in the MAPK signalling pathway PCR array as described in the Experimental section. Histograms show data for: (A) transcription factors, (B) cyclin-related proteins, (C) MAPKs and (D) other genes. All genes were normalized against five reference genes and data are expressed relative to control (PBS-treated) cells, and are means±S.E.M. for triplicate determinations from three independent experiments. Statistical analyses compared the HOSCN-treated samples with the no-oxidant (PBS-treated) control cells using one-way ANOVA with Bonferroni's post-hoc test, *P<0.05.