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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Environ Microbiol Rep. 2010 Aug 1;2(4):514–523. doi: 10.1111/j.1758-2229.2009.00077.x

Table 3.

Primers for SCOTS protocol and verification.

Name Sequence (5′-3′) Application
Xba random TGCTCTAGACGTCCTGATGGTT9(N) cDNA library
Xba TGCTCTAGACGTCCTGATGGTT cDNA amplification
Sal random ATATGTCGACTGAATTCCGTAGG9(N) cDNA library
Sal ATATGTCGACTGAATTCCGTAGG cDNA amplification
16S forward AGAGTTTGATCMTGGCTCAG rRNA cloning
23S reverse ATGGTTAAGCCTCACGGGCA rRNA cloning
speA sense GTTAAACCACGTCTTGGCTTGCGT Secondary verification
speA antisense ACCACCACCCACATCGAGGTATTT Secondary verification
Chitinase sense TCAGTCGGTGGATGGACGCT Secondary verification
Chitinase antisense GACGCTGATAATGGCGACAT Secondary verification
ilvD sense TCCGTCGAATACATGGTCAA Secondary verification
ilvD antisense TTTCAAACGCTGCTTTGTTG Secondary verification
ompW sense CCACCTACCTTTATGGTCC Secondary verification
ompW antisense GGTTTGTCGAATTAGCTTCACC Secondary verification
vibH sense TTGATGGCTACAGCTTGCAC Secondary verification
vibH antisense ATTGATCCACAGCGGTAAGG Secondary verification