Figure 1.

T-cell response of dm2 mice immunized with L^d 61–80 peptide emulsified in CFA. dm2 mice were injected subcutaneously in the hind foootpad with L^d 61–80 peptide (100 ug) emulsified in CFA. Ten days later, draining popliteal LN T cells were collected and tested for cytokine production by ELISPOT and for cytotoxic response against various targets. (A) T cells were cultured in vitro with the immunizing Ag (L^d 61–80, solid bars) or with a control Ag (HEL 46–61, hatched bars). Control non-immunized mice are shown as white bars. The results are presented as spots per million T cells + SD for each cytokine. The results are representative of five mice tested individually. CTL responses of mice immunized with L^d 61–80 peptide (B), no peptide (C), and control OVA 323–339 peptide (D). The ability of T cells to kill allogeneic L^d+ (BALB/c, squares), L^d (B6, circles) and control syngeneic (dm2, triangles) are shown. Spontaneous release ranged from 10 to 15% of maximal release. Data are representative of three independent experiments including 4–5 mice tested individually.