Table 1.

Adjuvant-driven T-cell responses of L^d 61–80 peptide in dm2 mice

Immunization
	none	Ld 61–80 CFA	Ld 61–80 IFA	Ld 61–80 soluble
IL-2	8	188	12	15
gIFN	5	224	8	12
IL-4	2	5	162	3
IL-5	2	2	98	4

dm2 mice were immunized subcutaneously with Ld 61–80 MHC class I allopeptide given with CFA, IFA or without adjuvant. Ten days later, CD4⁺ T cells were isolated from draining lymph nodes and cultured in vitro for 40 h with L^d 61–80 peptide. The frequencies of CD4⁺ T cells producing type 1 cytokines (IL-2 and IFN-γ) and type 2 cytokines (IL-4 and IL-5) were measured by ELISPOT. The results represent the number of spots per million CD4⁺ T cells. Bold underlined numbers represent values that are significantly higher than the background (p<0.001). The number of spots obtained with T cells from non-immunized mice and mice immunized with HEL 46–61 control peptide ranged from 0 to 4 spots per million T cells. The number of spots obtained with T cells from L^d 61–80 peptide-immunized mice cultured in the absence of peptide ranged from 2 to 5 spots per million T cells. The results are representative of three experiments performed on 2–4 mice tested individually.