Table 1.
Immunization | ||||
---|---|---|---|---|
none | Ld 61–80 CFA | Ld 61–80 IFA | Ld 61–80 soluble | |
IL-2 | 8 | 188 | 12 | 15 |
gIFN | 5 | 224 | 8 | 12 |
IL-4 | 2 | 5 | 162 | 3 |
IL-5 | 2 | 2 | 98 | 4 |
dm2 mice were immunized subcutaneously with Ld 61–80 MHC class I allopeptide given with CFA, IFA or without adjuvant. Ten days later, CD4+ T cells were isolated from draining lymph nodes and cultured in vitro for 40 h with Ld 61–80 peptide. The frequencies of CD4+ T cells producing type 1 cytokines (IL-2 and IFN-γ) and type 2 cytokines (IL-4 and IL-5) were measured by ELISPOT. The results represent the number of spots per million CD4+ T cells. Bold underlined numbers represent values that are significantly higher than the background (p<0.001). The number of spots obtained with T cells from non-immunized mice and mice immunized with HEL 46–61 control peptide ranged from 0 to 4 spots per million T cells. The number of spots obtained with T cells from Ld 61–80 peptide-immunized mice cultured in the absence of peptide ranged from 2 to 5 spots per million T cells. The results are representative of three experiments performed on 2–4 mice tested individually.