Table 1.

Histological findings of avascular necrosis of the femoral head (ANFH) in Wistar Kyoto rats

Sex	Treatment	No. of rats	OA/GC	OEL	EO	LO
Male	Placebo	6	2	1	1	1
Male	Prednisone	7	5	1	4	1
Female	Placebo	5	1	0	1	0
Female	Prednisone	12	3	2	1	2

The histological findings of an established (late stage) ANFH were defined as empty lacunae. Early signs of ANFH was considered when apoptosis occurred in more than three osteocytes and/or osteoblasts recognized in a high magnification field (×200); EO, number of early stages of osteonecrosis; LO, number of late stages of osteonecrosis; OA/GC, osteocyte apoptosis and/or ghost cell (a denucleated cell with an unstained center where the nucleus has been); OEL, osteocyte empty lacunae.