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. Author manuscript; available in PMC: 2010 Jul 29.
Published in final edited form as: J Proteome Res. 2008 Sep 4;7(10):4603–4608. doi: 10.1021/pr800456q

Figure 2.

Figure 2

Modification of acidic residues by glycerol. BSA was incubated in 50 mM ammonium bicarbonate, pH 8, containing 20% glycerol at 4 °C for 7 days before in-solution digestion by trypsin. The tryptic peptides were analyzed by HPLC/MS/MS in an LTQ mass spectrometer. (A) A peptide modified by glycerol at a glutamate residue. (B) A peptide modified by glycerol at an aspartate residue. (C) The number of glycerol-modified peptides from BSA identified in our analysis, in the presence or absence of glycerol incubation.