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. Author manuscript; available in PMC: 2010 Jul 29.
Published in final edited form as: J Neurosci Res. 2009 Jul;87(9):1997–2005. doi: 10.1002/jnr.22029

Fig. 4.

Fig. 4

Vitamin K1 and menaquinone-4 inhibit arachidonic acid-induced 12-lipoxygenase activation. A,B: The 12-LOX inhibitor AA861 prevented arachidonic acid-induced pre-OL death and ROS accumulation. Pre-OLs were treated as indicated with or without arachidonic acid (200 μM) in the absence or presence of AA861 (10 μM). Cell viability and ROS generation were evaluated 24 and 20 hr later, respectively. C: The 12-LOX-specific inhibitor AA861 blocked arachidonic acid-induced activation of 12-LOX and accumulation of its product 12-HETE. **P < 0.01 compared with controls. D: Vitamin K1 and MK-4 prevented arachidonic acid-induced 12-HETE accumulation in pre-OLs. Data are representative of three experiments. *P < 0.05, **P < 0.001 vs. the controls.