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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Nutr Cancer. 2010 Aug;62(6):811–824. doi: 10.1080/01635581003693082

Fig. 5. Comparison of the effects of CUR/CA and CUR/SIL combinations on the induction of apoptosis in HL-60 cells.

Fig. 5

A: Annexin-V binding assay. Cells were treated with the indicated polyphenols alone or in combination for 16 h followed by staining with FITC-conjugated annexin-V antibody and PI and flow cytometric analysis. Cells treated for 24 h with 10 μM Ara-C or 100 μM CA were used as the positive controls. B: Western blot analysis of PARP cleavage. Cells were treated with the indicated concentrations of polyphenols or Ara-C for 8 h and 24 h. Whole cell extracts were subjected to SDS-PAGE and immunoblotting. Representative data from 3 independent experiments are shown.