Fig. 3.

Pretreatment of TrHBMECs with ES936 inhibited TNFα-induced E-selectin protein expression. A, TrHBMECs were pretreated with ES936 (100 nM) for 2 h and then exposed to TNFα (10 ng/ml) for the indicated times. E-selectin protein levels were examined in TrHBMEC sonicates (15 μg) by nonreducing immunoblot analysis. β-Actin was included as a loading control. The blot is representative of at least three independent experiments. B, semiquantification (as described under Materials and Methods) of relative E-selectin protein levels after 4 h (peak protein level) of TNFα induction. n = 3; *, p < 0.05.