Fig. 4.

Pretreatment of TrHBMECs with ES936 inhibited TNFα-induced VCAM-1 and ICAM-1 protein expression. A, TrHBMECs were pretreated with ES936 (100 nM) or DMSO for 2 h and then exposed to TNFα (10 ng/ml) for the indicated times. VCAM-1 and ICAM-1 protein levels were examined in TrHBMEC extracts (15 μg) by immunoblot analysis. β-Actin was included as a loading control. The blot is representative of at least three independent experiments. B, semiquantification (as described under Materials and Methods) of relative VCAM-1 and ICAM-1 protein levels after 6 h (peak protein levels) of TNFα induction. n = 3; *, p < 0.05.