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. 2010 Jul;334(1):260–268. doi: 10.1124/jpet.110.167841

Fig. 6.

Fig. 6.

Inhibition or knockdown of NQO1 in TrHBMECs inhibited KG1a cell adhesion to TrHBMECs. KG1a (CD34+ hematopoietic) cells were perfused over TrHBMECs in a parallel plate flow chamber as described under Materials and Methods. Stimulation with TNFα (10 ng/ml for 4 h) significantly increased KG1a cell adherence to TrHBMECs (A, B, and D), whereas inhibition of NQO1 by ES936 dramatically decreased KG1a cell adhesion to TrHBMECs (B–D) in the presence of TNFα (10 ng/ml for 4 h) stimulation. Knockdown of NQO1 by siRNA also dramatically decreased TNFα (10 ng/ml, 4 h) induced KG1a cell adhesion to TrHBMECs (E). Results shown were determined from at least three independent experiments (n = 5 for ES936 pretreatment). *, p < 0.05. Arrows delineate smaller KG1a cells that have adhered to TrHBMECs.