Fig. 7.

Inhibition or knockdown of NQO1 did not inhibit TNFα-induced IκBα phosphorylation and degradation in TrHBMECs. A, TrHBMECs were transfected with siRNAs (either siRNA targeting NQO1 or scrambled siRNA control) for 72 h and then exposed to TNFα (10 ng/ml) for the indicated times. IκBα, phospho-IκBα, and NQO1 protein levels were examined in TrHBMEC extracts by immunoblot analysis. β-Actin was included as a loading control. The blot is representative of at least three independent experiments. B, TrHBMECs were pretreated with ES936 for 2 h and then exposed to TNFα (10 ng/ml) for the indicated times; IκBα and phospho-IκBα protein levels were examined in TrHBMEC extracts by immunoblot analysis. β-Actin was included as a loading control. The blot is representative of at least three independent experiments.