Fig. 8.

Inhibition or knockdown of NQO1 did not block TNFα-induced nuclear translocation of NF-κB subunit p65 in TrHBMECs. A, TrHBMECs were transfected with siRNAs (either siRNA targeting NQO1 or scrambled siRNA control) for 72 h and then exposed to TNFα (10 ng/ml) for the indicated times. Nuclear extracts were prepared, and p65 and NQO1 protein levels were examined by immunoblot analysis. PARP was included as a loading control. The blot is representative of at least three independent experiments. B, TrHBMECs were pretreated with ES936 for 2 h and then exposed to TNFα (10 ng/ml) for the indicated times. Nuclear extracts were prepared and p65 protein level was examined by immunoblot analysis. PARP was included as a loading control. The blot is representative of at least three independent experiments.