Fig. 9.

Inhibition or knockdown of NQO1 resulted in lower phospho-p65, c-Jun, and ATF2 levels in TrHBMEC nucleus in response to TNFα induction. A, TrHBMECs were transfected with siRNA (either siRNA targeting NQO1 or scrambled siRNA control) for 72 h and then exposed to TNFα (10 ng/ml) for the indicated times. Nuclear extracts were prepared, and phospho-p65, c-Jun, ATF2, and NQO1 protein levels were examined by immunoblot analysis. PARP was included as a loading control. The blot is representative of at least three independent experiments. Fold change is a same time point pairwise comparison between the control and siNQO1 treatment groups normalized to the PARP loading control. B, TrHBMECs were pretreated with ES936 for 2 h and then exposed to TNFα (10 ng/ml) for the indicated times. Nuclear extracts were prepared and phospho-p65, c-Jun, and ATF2 protein levels were examined by immunoblot analysis. PARP was included as a loading control. The blot is representative of at least three independent experiments. Fold change is a same time point pairwise comparison between the control and treatment groups normalized to the PARP loading control.