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. 2010 Jul;334(1):333–340. doi: 10.1124/jpet.110.166116

Fig. 6.

Fig. 6.

Effects of lubiprostone on morphine suppression of Isc, evoked by EFS were different for phases 1 and 2 of the evoked responses. Morphine (300 nM) suppressed phase 1 of the EFS-evoked Isc response and this action was reversed in concentration-dependent manner by application of lubiprostone in concentrations of 30 nm, 100 nM, and 300 nm after 5 min in the presence of morphine. The effect of lubiprostone on phase 2 in the presence of morphine was diametrically opposed to its action on phase 1. In the presence of morphine, lubiprostone suppressed phase 2 of the EFS-evoked responses in concentration-dependent manner. Transmural EFS was done with stimulus pulse parameters of 0.5-ms duration, 10-Hz frequency, and 3.5-mA strength. Values are expressed as means ± S.E.M., with n = 4 preparations from each of six animals (***, P < 0.001).