Fig. 1.
Protein levels of AC7 in pituitary gland. Levels of AC7 in whole pituitary glands of male (A) and female (B) Adcy7huTG, Adcy7(+/−), and WT mice were determined by immunoblotting as described under Materials and Methods. Each blot included tissue from male or female WT, Adcy7huTG, and/or Adcy7(+/−) mice, and the level of AC7 protein, quantitated as described in the text, was normalized to β-tubulin and expressed as percentage of WT on each blot. Percentage values were averaged across blots. Data are expressed as the mean ± S.E.M. from three to nine mice per group. *, P < 0.02 versus WT (two-way ANOVA and post-hoc contrasts). Representative blots are shown.