Fig. 3.
Effect of ethanol on plasma adrenocorticotropin levels. Male (left) and female (right) WT (red ■), Adcy7huTG (blue ♦), and Adcy7(+/−) (green ▲) mice were injected intraperitoneally with 2.25 g/kg ethanol and killed at the time points indicated. Adrenocorticotropin was measured by using an enzyme immunoassay kit as described in the text. Data are expressed as mean ± S.E.M. from four to eleven mice per group. *, P = 0.007, male Adcy7huTG compared with WT mice, and P < 0.001 compared with Adcy7(+/−) mice; **, P < 0.01, female Adcy7huTG compared with female Adcy7(+/−) mice; †, P < 0.002, female Adcy7huTG compared with Adcy7(+/−) mice. At 15, 45, and 75 min after ethanol injection, some groups of females had significantly higher adrenocorticotropin levels than males (see text).