TABLE 1.

Measures of pituitary levels of mRNA for adenylyl cyclase isoforms, CRF-1, G_sα protein, and proopiomelanocortin by microarray analysis

The percentage change in all cases represents the amount of difference in the quantity of mRNA for a particular transcript found in whole pituitary of the genetically modified strains versus the respective WT mice. All measurements were accomplished by using Affymetrics 430 v0.2 arrays, and data are included only if the probeset produced a “present” response (i.e., hybridization above background) on all the arrays (23) used in this experiment. P values were calculated by contrasting group means within a one-way ANOVA and were adjusted for multiple testing within gender by using a FDR.

Gene Symbol	Affymetrix Probe ID	Female Mice						Male Mice
		Adcy7(+/−)			Adcy7huTG			Adcy7(+/−)			Adcy7huTG
		Percentage Change	Unadjusted P Value	FDR	Percentage Change	Unadjusted P Value	FDR	Percentage Change	Unadjusted P Value	FDR	Percentage Change	Unadjusted P Value	FDR
		%			%			%			%
Adcy2	1455462_at	−5	0.451	0.650	−5	0.520	0.817	7	0.271	0.541	−1	0.828	0.876
Adcy3	1421960_at	14	0.027	0.293	10	0.135	0.744	−19	0.001	0.010	−23	<0.001	0.002
Adcy6	1418128_at	2	0.866	0.866	7	0.514	0.817	3	0.773	0.817	−2	0.810	0.876
Adcy7	1450065_at	−14	0.473	0.296	−17	0.438	0.916	−23	0.229	0.916	10	0.643	0.876
Adcy7	1456307_s_at	−35	0.054	0.650	−9	0.670	0.817	−20	0.290	0.817	3	0.876	0.876
Crhr1	1418810_at	2	0.788	0.866	−1	0.933	0.933	−16	0.060	0.933	5	0.555	0.876
Gnas	1450186_s_at	3	0.179	0.536	−3	0.337	0.780	0	0.921	0.921	−1	0.766	0.876
Gnas	1427789_s_at	−13	0.351	0.608	−30	0.042	0.374	−4	0.777	0.874	22	0.188	0.847
Pomc	1433800_a_at	3	0.411	0.650	1	0.775	0.916	−4	0.314	0.916	3	0.411	0.876