Western blot analysis of mIMCD-3 lysates. Western blot analyses of mIMCD-3 lysates (40 μg of total protein) were performed with commercially available anti-integrin antibodies to demonstrate the expression of these integrins on a protein level. The bands of α2 (155 kDa), α3 (150 and 130 kDa), α5 (150 kDa), α6 (140 kDa), αV (150 kDa), β1 (130 kDa), and β6 (97 kDa) are indicated.