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. 2010 Jul;78(1):126–134. doi: 10.1124/mol.110.064840

Fig. 3.

Fig. 3.

Transfection of mIMCD-3 cells with integrin and MMP siRNAs decreases BK-induced ERK activation. mIMCD-3 cells were nucleofected either with 100 nM siRNA for integrin α5β1 alone (-α5β1) or with combinations of MMP-8 siRNA (-α5β1-MMP-8) and/or MMP-13 siRNA (-α5β1-MMP-13), or with combinations of all siRNAs (-α5β1- MMP-8-MMP-13), or with the same amount of control siRNA (control). Forty-eight hours after nucleofection, cells were stimulated with vehicle or 100 nM BK (A) or with 1 ng/ml EGF (B) for 5 min, lysed, and analyzed for ERK phosphorylation. ERK phosphorylation was measured as described under Materials and Methods. Bars represent intensities of phospho-ERK bands relative to total ERK expressed as fold of basal (cells treated with vehicle). Experiments were performed three times in duplicate. Data are presented as mean + S.E.M. **, p < 0.01 compared with control BK-treated cells. ANOVA -α5β1 compared with -α5β1-MMP-8, -α5β1-MMP-13, or with -α5β1-MMP-8-MMP-13, not significant.